蔡雯,顾逸,张耀鹏,邵惠丽
CAI Wen,GU Yi,ZHANG Yao-peng,SHAO Hui-li

• 1:东华大学材料科学与工程学院 纤维材料改性国家重点实验室

摘要(Abstract)：

采用静电纺丝方法制备了再生丝素蛋白-羟丙基壳聚糖初生纤维毡并对其进行了乙醇后处理。通过扫描电镜、拉曼光谱、X射线衍射及各种性能分析手段研究了初生纤维毡和(或)后处理纤维毡的结构与性能。研究发现:随着羟丙基壳聚糖含量的增多,初生纤维毡中的纤维直径总体有减小的趋势,形貌由圆柱形纤维居多逐渐变成扁带状纤维居多,但仍以无规卷曲/α-螺旋构象和无定形结构为主,而后处理纤维毡的β-折叠构象含量、结晶度和力学性能均呈现先提高后下降的趋势,细胞增殖活性则逐渐增大,生物相容性随之提高。
In this paper, regenerated silk fibroin-hydroxypropyl chitosan(RSF-HPCS) composite mats wereprepared by electrospinning and then post-treated by ethanol. The structure and properties of the as-spunand(or) post- treated composite mats were characterized by SEM, Raman spectroscopy, XRD and othermethods. It was found that with the increase in the content of HPCS, the diameter of as-spun composite fi-bers decreased with the change in morphology from cylinder-like shape to ribbon-like shape. And randomcoil/α-helix conformation and amorphous structure were still predominant in as-spun mats. On the otherhand, with the addition of appropriate amount of HPCS, the post-treated mats showed higher crystallinityand β-sheet conformation content and their mechanical properties, biocompatibility and proliferation activi-ty were also improved.

关键词(KeyWords)： 丝素蛋白;羟丙基壳聚糖;静电纺丝;共混纤维
silk fibroin,hydroxypropyl chitosan,electrospinning,composite fiber

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(21274018,50803011,81170641);; 上海市教育委员会科研创新项目(12ZZ065);; 上海市青年科技启明星计划(12QA1400100);; 中央高校基本科研业务费专项资金资助

作者(Author): 蔡雯,顾逸,张耀鹏,邵惠丽
CAI Wen,GU Yi,ZHANG Yao-peng,SHAO Hui-li

DOI: 10.16090/j.cnki.hcxw.2014.11.008

参考文献(References)：

• [1]G H Altman,F Diaz,C Jakuba,et al.Silk-based biomaterials[J].Biomaterials,2003,24(3):401-416.
• [2]张幼珠,尹桂波,吴佳林,等.再生丝素/壳聚糖共混纳米纤维的结构与性能[J].合成纤维,2006,35(12):30-33.
• [3]W H Park,L Jeong,D I Yoo,et al.Effect of chitosan on morphology and conformation of electrospun silk fibroin nanofibers[J].Polymer,2004,45(21):7 151-7 157.
• [4]K H Zhang,Q Z Yu,X M Mo.Fabrication and intermolecular interactions of silk fibroin/hydroxybutyl chitosan blended nanofibers[J].International Journal of Molecular Sciences,2011,12(4):2 187-2 199.
• [5]P Chen,S H Chen,G J Lai.Preparation and characterization of biomimetic silk fibroin/chitosan composite nanofibers by electrospinning for osteoblasts culture[J].Nanoscale Research Letters,2012,7:170.
• [6]黄文良,邓江,阮世强,等.丝素蛋白/壳聚糖复合材料软骨组织工程支架的制备初探[J].遵义医学院学报,2008(6):581-583.
• [7]Y H Ruan,H Lin,J R Yao,et al.Preparation of 3D fibroin/chitosan blend porous scaffold for tissue engineering via a simplified method[J].Macromolecular Bioscience,2011,11(3):419-426.
• [8]袁毅桦,陈忻,刘颖梅,等.羟丙基壳聚糖的制备及其吸湿、保湿性研究[J].精细与专用化学品,2005(7):18-21.
• [9]陈忻,袁毅桦,张莉萍,等.羟丙基壳聚糖的制备[J].合成化学,2004(1):85-88.
• [10]范苏娜,张耀鹏,邵惠丽.静电纺丝素蛋白纤维毡在乙醇水溶液中的拉伸后处理[J].功能材料,2012(18):2 509-2 512.
• [11]L Zhou,X Chen,Z Z Shao,et al.Effect of metallic ions on silk formation the mulberry silkworm,Bombyx mori[J].Journal of Physical Chemistry B,2005,109(35):16 937-16 945.
• [12]A Glisovic,T Salditt.Temperature dependent structure of spider silk by X-ray diffraction[J].Applied Physics a-Materials Science&Processing,2007,87(1):63-69.
• [13]黄哲玮,孙皎,孟爱英.两种体外细胞毒性检测方法的比较研究[J].上海生物医学工程,2005(4):205-207.
• [14]尹桂波,张幼珠.静电纺再生丝素纳米纤维形态结构的研究[J].丝绸,2005(2):16-18.
• [15]H Pan,Y P Zhang,Y C Hang,et al.Significantly reinforced composite fibers electrospun from silk fibroin/carbon nanotube aqueous solutions[J].Biomacromolecules,2012,13(9):2 859-2 867.
• [16]S Koombhongse,W X Liu,D H Reneker.Flat polymer ribbons and other shapes by electrospinning[J].Journal of Polymer Science Part BPolymer Physics,2001,39(21):2 598-2 606.
• [17]A Pedicini,R J Farris.Mechanical behavior of electrospun polyurethane[J].Polymer,2003,44(22):6 857-6 862.
• [18]邹兆伟,史福军,黄宗海,等.纳米载银磷酸锆抗菌聚氨酯的细胞毒性检测[J].中国组织工程研究与临床康复,2011,15(38):7 149-7 152.
• [19]于同隐,梅娜,陈光,等.丝素蛋白在组织工程中的应用[J].复旦学报,自然科学版,2003,(6):828-832.