钟黎,张茗皓,盛楠,王宝秀,陈仕艳
ZHONG Li,ZHANG Ming-hao,SHENG Nan,WANG Bao-xiu,CHEN Shi-yan

• 1:东华大学纤维材料改性国家重点实验室东华大学材料科学与工程学院

摘要(Abstract)：

海藻酸钙水凝胶敷料作为一种"湿疗法"产品广泛用于伤口护理领域,但其无抗菌性,且缺乏细胞黏附的位点。通过将海藻酸盐和纳米氧化细菌纤维素(TOBC)共混,使用浸渍富集法负载抗菌剂聚六亚甲基双胍(PHMB)制备出一种多功能复合水凝胶,并使用场发射扫描电镜、酶标仪、激光共聚焦显微镜对复合水凝胶的结构和性能进行表征。结果表明:复合水凝胶为透明状,表面存在大量的纳米纤维,生物活性得到提高。使用0.001%PHMB溶液处理后,复合水凝胶对于大肠埃希菌和金黄葡萄球菌均具有良好抗菌效果,同时兼具优异的生物相容性。
Calcium alginate hydrogel dressing is widely used in the field of wound care as a "wet therapy" product, but it has no antibacterial property and lacks sites for cell adhesion. In this paper, a kind of multifunctional composite hydrogel was prepared by blending alginate and nano-oxidized bacterial cellulose(TOBC) and loading antibacterial agent PHMB. The structure and properties of the composite hydrogel were characterized by means of SEM, microplate reader and laser confocal microscope. The results showed that the composite hydrogel was transparent and had a large number of nanofibers on its surface,which improved its biocompatibility. After treatment with 0.001% PHMB solution, the composite hydrogel had good antibacterial effect on both Escherichia coli and Staphylococcus aureus, and also had excellent biocompatibility.

关键词(KeyWords)： 海藻酸钙;复合水凝胶;抗菌;生物相容性;PHMB
calcium alginate;composite hydrogel;antibacterial;biocompatibility;PHMB

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 钟黎,张茗皓,盛楠,王宝秀,陈仕艳
ZHONG Li,ZHANG Ming-hao,SHENG Nan,WANG Bao-xiu,CHEN Shi-yan

DOI: 10.16090/j.cnki.hcxw.2019.05.001

参考文献(References)：

• [1]GUERRA A,BELINHA J,JORGE R N.Modelling skin wound healing angiogenesis:Areview[J].Journal of Pharmaceutical and Biomedical Analysis,2019,163:1-17.
• [2]CONG Z,SHI Y,WANG Y,et al.A novel controlled drug delivery system based on alginate hydrogel/chitosan micelle composites[J].International Journal of Biological Macromolecules,2017,107:308-317.
• [3]PISTONE S,QORAGLLU D,SMISTAD G,et al.Formulation and preparation of stable cross-linked alginate-zinc nanoparticles in the presence of a monovalent salt[J].Soft Matter,2015,11(28):5765-5774.
• [4]TURCO G,MARSICH E,BELLOMO F,et al.Alginate/hydroxyapatite biocomposite for bone ingrowth:a trabecular structure with high and isotropic connectivity[J].Biomacromolecules,2009,10(6):1575-1583.
• [5]PAWAR S N,EDGAR K J.Alginate derivatization:A review of chemistry,properties and applications[J].Biomaterials,2012,33(11):3279-3305.
• [6]VENKATESAN J,KIM D G,KANG D S,et al.Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles[J].International Journal of Biological Macromolecules,2017,98:515-525.
• [7]HU Y,ZHANG Z Y,LI Y,et al.Dual-crosslinked amorphous polysaccharide hydrogels based on chitosan/alginate for wound healing applications[J].Macromolecular Rapid Communications,2018,39(20):306-312.
• [8]YANG K R,HAN Q,CHEN B P,et al.Antimicrobial hydrogels:promising materials for medical application[J].International Journal of Nanomedicine,2018,13:2217-2263.
• [9]VENKATESAN J,LEE J Y,KANG D S,et al.Antimicrobial and anticancer activities of porous chitosan-alginate biosynthesized silver nanoparticles[J].International Journal of Biological Macromolecules,2017,98:515-525.
• [10]NAPAVICHAYANUN S,YAMDECH R,ARAMWIT P.The safety and efficacy of bacterial nanocellulose wound dressing incorporating sericin and polyhexamethylene biguanide:in vitro,in vivo and clinical studies[J].Archives of Dermatological Research,2016,308(2):123-132.
• [11]MATTOS I B,HOLZER J C J,TUCA A C,et al.Uptake of PHMB in a bacterial 11 nanocellulose-based wound dressing:a feasible clinical procedure[J].Burns:Journal of the International Society for Burn Injuries,2018,10:23-26.
• [12]CARNEIRO B M L,WOPEREIS D B,FABIANA C,et al.Contact lens-related polymicrobial keratitis:acanthamoeba spp.genotype t4 and candida albicans[J].Parasitology Research,2018,117(11):3431-3436.
• [13]HOU Y,WANG X,YANG J,et al.Development and biocompatibility evaluation of biodegradable bacterial cellulose as a novelperipheral nerve scaffold[J].Journal of Biomedical Materials Research Part A,2018,106(5):32-36.
• [14]RAAFAT A I,EL S N,BADAWY N A,et al.Radiation fabrication of xanthan-based wound dressing hydrogels embedded zno nanoparticles:in vitro evaluation[J].International Journal of Biological Macromolecules,2018,118:1892-1902.
• [15]ZHANG T,WANG W,ZHANG D,et al.Bacteria cellulose nanofiber nanocomposites and their application in biosensing[J].Advanced Functional Materials,2010,20(7):1152-1160.